The effects of baicalein on promoting the osteogenesis of human periodontal ligament cells
CHEN Lijiao1,YU Wenbin1, REN Manman2, CEN Shengdan2, DENG Hui2, HU Rongdang1
1.Department of Orthodontics,Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027; 2.Department of Periodontics, Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027
CHEN Lijiao,YU Wenbin,REN Manman, et al. The effects of baicalein on promoting the osteogenesis of human periodontal ligament cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(1): 7-12.
Abstract：Objective: To evaluate the effects of baicalein extracted from Scutellaria baicalensis georgi on the function of proliferation and osteogenesis of human periodontal ligament cells (hPDLCs). Methods:Baicalein at a concentration of 0.3125, 0.625, 1.25 μmol/L was added into hPDLCs primary cultured in vitro accordingly as experimental group, the one with no baicalein as blank control group. The effect of proliferation of hPDLCs was assessed through MTT assay. Alkaline phosphatase (ALP) activity assay, Alizarin red S staining,RT-qPCR and Western Blot were used to observe the ALP activity, formation of mineral nodules, mRNA and protein expression of collagenase-Ι (Col-Ι) of hPDLCs, respectively. Results: Compared to the control group,baicalein at different concentrations had no significant influence on the proliferation of hPDLCs. Baicalein was shown to enhance the ALP activity of hPDLCs. After 21 days of osteogenic induction, more mineral nodules could be observed compared to the control group, which increased in a dose-dependent manner at the concentration of 0.3125-1.25 μmol/L baicalein according to the results of quantification. The mRNA and protein level of Col-Ι increased by baicalein in different concentration, which reached maximum at 7 days, appearring in a dosedependent manner from 0.3125 to 1.25 μmol/L using Western Blot and Real-time PCR analysis. Conclusion:Baicalein can promote hPDLCs to differentiate into osteoblasts, which is of great value for further study acting as the adjuvant drug for periodontal regenerative therapy.
