The role of RAGE-ROS axis in the apoptosis of gingival fibroblast in diabetic periodontitis
HUANG Shengbin, DAI Panpan, MAO Yixin, LI Xumin, MA Jianfeng. Department of Prosthodontics, Hospital of Stomatology Wenzhou Medical University, Wenzhou, 325027
Shengbin,DAI Panpan,MAO Yixin, et al. The role of RAGE-ROS axis in the apoptosis of gingival fibroblast in diabetic periodontitis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(12): 864-.
Abstract：Objective: To investigate the role of RAGE-ROS axis in the apoptosis of gingival fibroblast in diabetic periodontitis. Methods: Thirty wistar rats (male, six weeks old) were randomly divided into two groups: normal control group (N) and diabetic periodontitis group (D). Four weeks later, animals were sacrificed; meanwhile, the alveolar bone loss and apoptosis of gingival fibroblast were detected while the expression of RAGE and oxidative stress marker were also investigated. Results: Compared to N group, more severe alveolar bone loss, along with more apoptosis of gingival fibroblast appeared in D group (P<0.05). The expression of RAGE and oxidative stress marker increased significantly in gingival tissue in D group (P<0.05). Conclusion: RAGE-ROS axis plays an important role in the apoptosis of gingival fibroblast in diabetic periodontitis.
[1]Taylor JJ, Preshaw PM, Lalla E. A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes[J]. Clin J Periodontol, 2013, 40 suppl 14: S113-134.
[2]Desta T, Li J, Chino T, et al. Altered fibroblast proliferation and apoptosis in diabetic gingival wounds[J]. J Dent Res,2010, 89(6): 609-614.
[3]International Diabetes Federation. (2012) IDF Diabetes Atlas[R]. 5th ed. International Diabetes Federation, 2012.
[4]Cipollone F, Iezzi A, Fazia M, et al. The receptor RAGE as a progression factor amplifying arachidonate-dependent inflammatory and proteolytic response in human atherosclerotic plaques: role of glycemic control[J]. Circulation, 2003, 108(9): 1070-1077.
[5]Ramasamy R, Vannucci SJ, Yan SS, et al. Advanced glycation end products and RAGE: a common thread in aging, diabetes, neurodegeneration, and inflammation[J]. Glycobiology, 2005, 15(7): 16-28.
[6]Katz J, Bhattacharyya I, Farkhondeh-Kish F, et al. Expression of the receptor of advanced glycation end products in gingival tissue of type 2 diabetes patients with chronic periodontal disease: a study utilizing immunohistochemistry and RT-PCR[J]. J Clin Periodontol, 2005, 32(1): 40-44.
[7]Thomas MC, Forbes JM, Cooper ME. Advanced glycation end products and diabetic nephropathy[J]. Am J Ther, 2005, 12(6): 562-572.
[8]Schmidt AM, Weidman E, Lalla E, et al. Advanced glycation end products (AGEs) induce oxidant stress in the gingiva: a potential mechanism underlying accelerated periodontal disease associated with diabetes[J]. J Periodontal Res, 1996, 31 (7): 508-515.
[9]Takeda M, Ojima M, Yoshioka H, et al. Relationship of serum advanced glycation end products with deterioration of periodontitis in type 2 diabetes patients[J]. J Periodontol, 2006, 77(1): 15-20.
[10]Govindaraj P, Khan NA, Gopalakrishna P, et al. Mitochondrial dysfunction and genetic heterogeneity in chronic periodontitis [J]. Mitochondrion, 2011, 11(3): 504-512.
[11]Graves DT, Liu R, Alikhani M, et al. Diabetes enhanced inflammation and apotosis-impact on periodontal pathology[J]. J Dent Res, 2006, 85(1): 15-21.
[12]付永伟, 和红兵, 欧炯光. 实验性糖尿病牙周炎诱导骨细胞凋亡的初步研究[J]. 现代口腔医学杂志, 2009, 23(5): 500-504.
[13]付永伟, 和红兵, 欧炯光. 实验性糖尿病牙周炎牙周膜中成纤维细胞凋亡的初步研究[J]. 实用口腔医学杂志, 2010, 26(3): 310-314.
[14]邓天政, 吕晶, 逢键梁, 等. 细胞凋亡在糖尿病大鼠牙周炎中参与牙槽骨吸收的实验研究[J]. 北京口腔医学, 2010, 18(5): 257-260.
