Ultrasound-microbubble mediated heparin-poloxamer thrombolysis study in vitro
1.Department of Pharmacy, Traditional Chinese Medicine Hospital of Zhejiang, Hangzhou, 310006; 2.School of Pharmacy, Wenzhou Medical University, Wenzhou, 325035
WANG Yan1,ZHAO Yingzheng2,TANG Qinqin2.. Ultrasound-microbubble mediated heparin-poloxamer thrombolysis study in vitro[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(11): 823-.
Abstract：Objective: To explore and discuss the mechanism of theapeutical ultrasound combined with heparin-poloxamer (HP) on thrombus dissolution in vitro. Methods: Blood clots were prepared from rabbit ear blood. Main factors on the thrombolysis effects in vitro were investigated, including blood clot age, ultrasonic intensity (pure intensity seted in the range of 2~8 W/cm2), ultrasound time and material concentration. Five experimental groups were as follows: ①US; ②US+MB; ③HP; ④US+HP; ⑤US+MB+HP. Through the results of the experiment, thrombolysis effect in each group was determined and the mechanism was discussed. Results: The group (US+MB+HP) showed the best result in the thrombolysis with less ultrasound time, though the other groups showed certain thrombosis effects in vitro. Conclusion: Heparin-poloxamer combined with ultrasound microbubble has an improved effect in thrombolysis, which may be a potential technique for clinical treatment of thrombotic diseases.
