The construction of SW480 cells nude model transfected by hNIS gene and radionuclide imaging
1.Department of Nuclear Medical, Wenzhou Medical University, Wenzhou, 325035; 2.Depaertment of Nuclear Medical, SIR RUN RUN SHAW Hospital, Zhejiang University, Hangzhou, 310020; 3.Radiological Department, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
ZHANG Hongzheng1,DU Fan2,ZHOU Bailin3, et al. The construction of SW480 cells nude model transfected by hNIS gene and radionuclide imaging[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(11): 803-.
Abstract：Objective: To examine the feasibity of radionuclide imaging mediated by colon cancer cells (SW480) nude model transfected with a recombinant pcDNA3.1+-hNIS plasmid. Methods: pcDNA3.1+-hNIS was confirmed by restriction enzyme digestion, the hNIS gene and protein expression were detected after transfection, the SW480 cells of stably expressing hNIS were screened, the tumors nude mice model was established, then radioactive nuclide 99mTcO4- imaging was obtained. Results: After SW480 cells was transfected with pcDNA3.1+-hNIS, the hNIS gene and prote in expression were successfully detected. Radionuclide imaging was successfully mediated by colon cancer cells nude model. Conclusion: The hNIS expression can be achieved in colon nude model by the recombinant plasmid pcDNA3.1+-hNIS, and make the radionuclide imaging and radionuclide therapy of colon cancer as possible.
[1] Jemal A, Bray F, Center MM, et al. Global cancer statistics [J]. CA Cancer J Clin, 2011, 61(2): 69-90.
[2] Kim YH, Youn H, Na J, et al. Codon-optimized human sodium iodide symporter (opt-hNIS) as a sensitive reporter and efficient therapeutic gene[J]. Theranostics, 2015, 5(1): 86-96.
[3] Terzić J, Grivennikov S, Karin E, et al. Inflammation and Colon Cancer[J]. Gastroenterology, 2010, 138(6): 2101-2114.
[4] Smit JW, Shroder-Van DEJ, Karperien M, et al. Reestablishment of in vitro and in vivo iodide uptake by transfection of the human sodium iodide symporter (hNIS) in a hNIS defective human thyroid carcinoma cell line[J]. Thyroid, 2000, 10(11): 939-943.
[5] 赵兴业, 李玮, 谭建, 等. hTERT启动子引导hNIS基因重组腺病毒介导放射性核素的裸鼠显像[J]. 天津医科大学 学报, 2012, 18(4): 422-424.
[6] Gao XF, Zhou T, Chen GH, et al. Radioiodine therapy for castration-resistant prostate cancer following prostate-specific membrane antigen promoter-mediated transfer of the human sodium iodide symporter[J]. Asian J Androl, 2014, 16(1): 120-123.
[7] Faivre J, Clerc J, Gerolami R, et al. Long-term radioiodine retention and regression of liver cancer after sodium iodide symporter gene transfer in wistar rats[J]. Cancer Res, 2004,64(21): 8045-8051.
[8] Dwyer RM, Bergert ER, O’Connor MK, et al. In vivo radioiodide imaging and treatment of breast cancer xenografts after MUC1-driven expression of the sodium iodide symporter[J]. Clin Cancer Res, 2005, 11(4): 1483-1489.
[9] Yan Y, Zhang H, Zhang Y, et al. Transfection of the human sodium/iodide symporter (NIS) gene with liposomes and the expression of the NIS protein in human lung A549 cancer cells[J]. Chin J Clin Oncol, 2008, 5(1): 30-34.
[10] Zuckier LS, Dohan O, Li Y, et al. Kinetics of perrhenate uptake and comparative biodistribution of perrhenate, pertechnetate, and iodide by NaI symporter-expressing tissues in vivo[J]. J Nucl Med, 2004, 45(3): 500-507.
[11] Scholz IV, Cengic N, Baker CH, et al. Radioiodine therapy of colon cancer following tissue-specific sodium iodide symporter gene transfer[J]. Gene Ther, 2005, 12(3): 272-280.
[12] 杜凡, 周佰林, 张红征, 等. 人钠/碘转运体基因转染结肠癌SW480细胞介导放射性碘治疗的实验研究[J]. 温州医科大学学报, 2014, 44(5): 334-337.
