Antimicrobial activity of tannic acid and its effect on biofilms of clinical isolates
1.School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, 325035; 2.Department of Laboratory Medicine, the Frist Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
YU Xiao1,HOU Yuanbo1,ZHOU Tieli2, et al. Antimicrobial activity of tannic acid and its effect on biofilms of clinical isolates[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(10 ): 709-.
Abstract：Objective: To analyze the antimicrobial activity of tannic acid against clinical isolates and its effect on biofilms. Methods: Nine kinds of common clinical isolates, including Staphylococcus aureus, Staphylococcus epidermidis, Hemolytic staphylococci, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii were collected. All kinds of antimicrobial agents were evaluated with agar dilution method for the minimal inhibitory concentrations (MIC). According to the results of susceptibility testing, 9 kinds of clinical isolates were divided into multidrug-resistant (MDR) group or non-multidrug-resistant group (nMDR). Thirty strains from each group were randomly selected as experimental strains. Agar dilution method was used to detect tannic acid MIC of the strain. The difference of the 2 groups to reflect the tannic acid antibacterial activities of common clinical isolates was compared. The effect on the biofilm formation was detected by adding tannic acid into the biofilm culture medium. Results: Tannic acid showed strong antibacterial activity to the 9 kinds of clinical isolates, antibacterial activity to the Staphylothermus bacteria was significantly greater than those for the other bacteria (P<0.05). Tannic acid could inhibit the bacterial biofilm formation. Conclusion: Tannic acid has antibacterial effect to common clinical isolates and can influence the formation of biofilm.
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