Levosimendan improves survival rate in lipid-epinephrine based resuscitation in a rat model of bupivacaine-induced cardiac arrest
1.Department of Anesthesiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Anesthesiology, the Medical Center of the Ohio State University, Columbus, 43085
NAN Fubei1,WU Bingjing1,DONG Jiaojiao1, et al. Levosimendan improves survival rate in lipid-epinephrine based resuscitation in a rat model of bupivacaine-induced cardiac arrest[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(8): 556-.
Abstract：Objective: To observe the effect of levosimendan on resuscitation using lipid combined with epinephrine in a rat model of bupivacaine-induced cardiac arrest. Methods: Twenty-four adult SD male rats were randomly divided into two groups: lipid in combination with both epinephrine and levosimendan (LLE, n=12)or lipid in combination with epinephrine (LE, n=12). Bupivacaine was injected intravenously to establish a rat model of cardiac arrest. Then regular external chest compression was started, and lipid emulsion combined with a small dose of epinephrine resuscitation was used for resuscitation. Additionally, the experimental group was treated with levosimendan, when the control group was given 0.9% sodium chloride. As our primary outcome was the survival rate at 45 mins, cases of successful resuscitation and rats survived to 45 mins were recorded. Besides, the time to the first heart beat and restoration of spontaneous circulation (ROSC), the amount of epinephrine, the wet to dry ratio of the lung, and the arterial blood gas data at the end of the balance period and at 45 min were also calculated. Results: The survival rate at 45 min in the LLE group was higher than that in the LE group (P<0.05). The time to the first heart beat and ROSC in the LLE group was shorter than that in the LE group (P<0.05). The amount of epinephine in LLE group was less than that in the LE group (P<0.05). The arterial blood gas data such as PH, BE, PaCO2 and PaO2 in the LLE group were all better than those in the LE group at 45 mins (P<0.05). Conclusion: In the rat model of lipid-epinephrine based resuscitation from bupivacaine-induced cardiac arrest, the combination with levosimendan improves the survival rate and accelerates the recovery.
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