Antifeeding and toxic activity of ethyl acetate extract from barks of Melia azedarach L against Spodoptera litura larvae
1.School of Pharmacy, Wenzhou Medical University, Wenzhou, 325035; 2.Institute of Healthy & Environmental Ecology, Wenzhou Medical University, Wenzhou, 325035
GE Junjun1,CHEN Pian1,HUANG Yilu1, et al. Antifeeding and toxic activity of ethyl acetate extract from barks of Melia azedarach L against Spodoptera litura larvae [J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(8): 552-.
Abstract：Objective: In order to find new compounds with strong antifeeding activities, the crude extract and fractions from barks of Melia azedarach L. were selected for their antifeeding and toxic activity against Spodoptera litura larvae. Methods: The 95% ethanol extract of the barks of Melia azedarach L. was concentrated, and the aqueous solution was partitioned with ethyl acetate. The ethyl acetate extract was subjected to silica gel CC eluted with ether-ethyl acetate to give 15 fractions (K1～K15). The fractions were investigated against Spodoptera litura larvae by leaf disc method to test the leaf area and the mortality. Results: Ethyl acetate extract from barks of Melia showed significant antifeeding activity against Spodoptera litura larvae, which was improved with increased dosages. Fractions 5, 6, 10 displayed strong toxic effects, and Fraction 11 also indicated toxic effects. Fractions 5, 6 revealed strong antifeeding activity even at the low concentration. Conclusion: Fractions 5, 6 demonstrate obvious antifeeding and toxic activity against Spodoptera litura larvae than Fractions 10, 11. The fractions which reflect stronger antifeeding activity will be isolated and purifies in hoping of achieving the new active compounds.
