EIF5A2在结直肠癌细胞中的表达及在5-Fu化疗抵抗中的作用

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摘要目的：探讨EIF5A2在结直肠癌细胞中的表达及在化疗抵抗中的作用。方法：培养对数生长期的人结直肠癌细胞系LOVO、SW480及DLD1，用Western blot法测定三种结直肠癌细胞系中EIF5A2的表达。将人结直肠癌LOVO细胞在浓度为0.2μg/L的5-Fu中培养48 h，用Western blot法测定EIF5A2的表达。EIF5A2-siRNA转染LOVO细胞，将转染后的LOVO细胞及结直肠癌LOVO细胞在0.2μg/L的5-Fu中培养48 h，用MTT法测定细胞生长抑制率。结果：EIF5A2在三种结直肠癌细胞系中均有表达，在LOVO细胞中表达量最高（P＜0.05）。结直肠癌LOVO细胞在5-Fu中培养48 h后 EIF5A2表达明显增强（P＜0.05）。EIF-5A2-siRNA转染LOVO细胞后增加了5-Fu的细胞抑制率（P＜0.05）。结论：EIF5A2在人结直肠癌细胞中有表达，并且增强了5-Fu的化疗抵抗力。

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	范永胂1
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关键词 ：结直肠肿瘤, EIF5A2, 5-氟尿嘧啶, 化疗抵抗

Abstract：Objective: To research EIF-5A2 overexpression in colorectal cancer and its effect on chemotherapy. Methods: EIF5A2 expression in colorectal carcinoma (CRC) line (LOVO, SW48 and DLD1) was measured by Western-blot. LOVO cells were cultured in 5-Fu for 48 hours, and EIF5A2 expression was measured by Western-blot. LOVO cells were treated with EIF5A2-siRNA and exposed to 0.2 μg/L 5-Fu for 48 hours. Cell toxicity was assayed by MTT. Results: EIF5A2 was expressed in all three different CRC and was highest in LOVO cells (P<0.05). 5-Fu treatment led to elevated EIF5A2 expression (P<0.05). The transfection of EIF5A2-siRNA could improve the inhibit rate of LOVO to 5-Fu (P<0.05). Conclusion: EIF5A2 is expressed in CRC lines and it can enhance the chemoresistance of 5-Fu.

Key words： colorectal neoplasm EIF5A2 5-Fu chemoresistance

收稿日期: 2014-08-07

作者简介: 范永胂（1980-），男，浙江杭州人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2015/V45/I7/516

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