姜黄素通过下调p-NF-κB表达减轻大鼠带状疱疹神经痛

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摘要目的：观察姜黄素对大鼠带状疱疹神经痛动物模型行为学及脊髓背角和背根神经节（DRG）的磷酸化核因子-κB（p-NF-κB）的影响，探讨姜黄素用于带状疱疹神经痛治疗的疗效及其机制。方法：选取雄性SD大鼠80只，随机分为4组（各20只）：C组、D组、Dcur1组、Dcur2组。C组：后爪注射0.9%氯化钠溶液；D组：将单纯疱疹病毒-1（HSV-1）接种于大鼠后爪，5 d后行0.9%氯化钠溶液腹腔注射治疗。Dcur1组、Dcur2组：HSV-1接种大鼠5 d后行腹腔内姜黄素注射治疗，剂量分别为100 mg/kg、200 mg/kg。于造模前、造模后5 d（治疗前）、治疗结束后1、7、14 d，测定机械缩足阈值（MWT）、热缩足潜伏期（TWL）；于治疗结束后14 d检测脊髓和DRG中p-NF-κB表达。结果：大鼠感染HSV-1后MWT从43.8降低到15.0，TWL从10.2降低到6.7，脊髓背角p-NF-κB表达从1.3增加到26.1，DRG中p-NF-κB表达从0.7增加到12.1。2组大鼠注射姜黄素后的MWT和TWL均明显增加，而p-NF-κB的表达均明显降低（Dcur1组脊髓背角和DRG分别降低到5.7和4.8，Dcur2组脊髓背角和DRG分别降低到3.1和2.0）。结论：姜黄素早期注射能明显缓解带状疱疹神经痛，其机制与下调脊髓背角和DRG的p-NF-κB过表达有关。

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	皮治兵1
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关键词 ：带状疱疹神经痛；姜黄素；磷酸化核因子-&kappa, B；大鼠

Abstract：Objective: To evaluate the effect of curcumin on herpetic neuralgia in rats and its mechanisms. Methods: Sixty rats with therpetic neuralgia induced by claw injection of HSV-1 and 20 healthy control (C group) rats whose claw were injected by normal saline were included. Twenty rats which had been injected HSV-1 were injected in intraperitoneal 100 mg/kg curcumin 5 days later (Dcur1 group), and 20 rats were injected 200 mg/kg curcumin (Dcur2 group), and the rest 20 rats were injected normal saline (D group ). We tested mechanical allodynia with electronic von frey and tested thermal hyperalgesia with radiant heat before HSV-1 injection, 5 days later after HSV-1 injection, on 1, 7, 14 days after curcumin or NS injection. p-NF-κB in lumbar spinal cord (L4~5) and DRG was estimated by immunohistochemistry at 14 d after NS or curcumine was given. Results: Compared to C group, Shingles rats exhibited significant pain hypersensitivity, as evidenced by decreased paw withdrawal threshold to electronic von Frey and decreased paw withdrawal latency to heat. After herpes infection, rat’s mechanical paw withdrawal threshold reduced from 43.8 to 15.0, and thermal withdrawal latencies reduced from 10.2 to 6.7. Phosphorylation of nuclear factor (p-NF)-κB expression in the spinal cord dorsal horn increased from 1.3 to 26.1, while phosphorylation of nuclear factor (p-NF)-κB expression in the dorsal root ganglion increased from 0.7 to 12.1. However, curcumine significantly attenuated the Shingles-induced allodynia and hyperalgesia and reduced the expression of p-NF-κB (Dcur1 spinal cord dorsal horn/dorsal root ganglion reduced to 5.7/4.8, Dcur2 spinal cord dorsal horn/dorsal root ganglion reduced to 3.1/2.0). Conclusion: Curcumine seems attenuate pain threshold in herpetic neuralgia rats, possibly through an inhibitory action on p-NF-κB expression in spinal dorsal horn and dorsal root ganglion.

Key words： herpetic neuralgia curcumine p-NF-κB rats

收稿日期: 2014-12-29

基金资助:浙江省医药卫生平台骨干人才计划项目（2011RCB026）。

通讯作者: 林海，副教授，硕士生导师，Email：422133061@qq.com。

作者简介: 皮治兵（1977-），男，湖北通城人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2015/V45/I7/512

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