XIAO Fanglan,LV Panpan,YAN Xijuan, et al. The establishment of a specific E.coli for detection of mercury (II) ions[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(7): 474-.
Abstract：Objective: To construct a specific biosensor to detect mercury ions using the enhanced cyan fluorescent protein as a reporter. Methods: The fusion reporter vector, merR-O\P-ecfp-T, was constructed using ecfp as reporter gene. Then the reporter vector merR-O\P-ecfp-T was transformed into Escherichia coli MC4100 wide type strain. The growth conditions optimization of this completed biosensor and its specificity and the optimum detection range parameters were determined. Results: The results showed that the mercury biosensor had a good specificity, the optimal detection range of mercury concentration was 0.15-38.4 μmol/L. A specific biosensor were successfully constructed to detect mercury ions. Conlusion: The work provides the foundation for developing other hypersensitive biosensors for the chemicals such as explosives, polycyclic aromatic hydrocarbons, polychlorinated hydrocarbons and heavy metals that are important to national security, human health, environment, energy supply and food safety.
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