Monitor of air borne hospital environment by Illumina high-throughput sequence
1.Department of Respiratory and Critical Care Medicine, Beijing Hospital, Beijing, 100730; 2.Department of Geriatrics, Beijing Hospital, Beijing, 100730; 3.Department of Laboratory Medicine, Beijing Hospital, Beijing, 100730; 4.Center of Biomedical Analysis, School of Life Sciences, Tsinghua University, Beijing, 100084; 5.Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Beijing, 100730; 6.Department of Hospital Infection Control and Management, Beijing Hospital, Beijing, 100730
LI Yanming1,6,TONG Xunliang2, et al. Monitor of air borne hospital environment by Illumina high-throughput sequence[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(6): 437-.
Abstract：Objective: To monitor microorganisms in air borne environment in hospital and to analysis the distribution characters of microorganisms in different departments in hospital. Methods: Air samplers were utilized in four typical departments of hospital for airborne microorganisms’ collection. Microorganisms were identified by DNA sequencing analysis by Illumina MiSeq and HiSeq 2000 sequencing systems. Results: The airborne microorganisms from four departments were analyzed for microbiome sequencing and classified as fungi,species, bacteria species and viruses species. The ratio of pathogenic fungi in fungi was different in four departments and the ratio in two intensive care units were higher than its in outpatient department and emergency department. At the species level of fungi, ratio of yeast, mycotic fungus and dimorphic fungi in different department was shown. The relative ratio of aspergillus in mycotic fungus and the relative ratio of cryptococcus and candida in yeast was shown,respectively. Conclusion: Novel method was utilized in monitoring the air borne microorganisms in hospital environment and the relative ratio of common opportunistic pathogenwas satisfied. It is provided experiment based information in improving the methodology in monitoring hospital infection.
