XIANG Zhenfei,LU Miaozhen,ZHANG Huanle, et al. The epigenetic alteration and function of PLAGL1 in colorectal cancer[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(6): 430-.
Abstract：Objective: Epigenetic alterations have played an important role in colorectal carcinogenesis. PLAGL1 (pleiomorphic adenoma gene-like 1, Also known asZAC; LOT1; ZAC1) is a gene associated with the development of many types of cancers. Our aims are to explore the expression and the function of PLAGL1 in colorectal cancer. Methods: RT-PCR (semi-quantitative reverse-transcription PCR) was used in this study to detect the expression level of PLAGL1 in colorectal cancer cell lines. The methylation of PLAGL1 promoter regain was detected with MSP (methylation specific PCR) in colorectal cancer cell lines and colorectal cancer tissues. MTT assay, colony formation and cell cycle analysis were used to explore the function of PLAGL1 in colorectal carcinogenesis. Results: The expression of PLAGL1 in colorectal cancer cell lines was regulated by promoter regain methylation. And PLAGL1 was frequently methylated in 64.4% (67/104) colorectal cancer tissue. Restoration of PLAGL1 expression in HCT116 inhibited cell proliferation and colony formation, and cell cycle of HCT116 was arrested at G1 phase. Conclusion: The expression of PLAGL1 in colorectal cancer is regulatied by DNA methylation.The restoration of PLAGL1 can suppressed the proliferation of colorectal cancer cell HCT116, and also arrest the cell cycle of HCT116 in G1 phase. PLAGL1 acts as a tumor suppressor in colorectal carcinogenesis.
