Effects of extract of Ginkgo biloba on apoptosis and the expression of Drp-1, Mfn-2 in myocardial cells induced by high glucose and its underlying mechanism
Department of Pathology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
LI Jianmin,WU Ling,ZHANG Di, et al. Effects of extract of Ginkgo biloba on apoptosis and the expression of Drp-1, Mfn-2 in myocardial cells induced by high glucose and its underlying mechanism[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(6): 397-.
Abstract：Objective: To investigate the effects of Ginkgo biloba on apoptosis and the expression of Drp-1, Mfn-2, Bax, Bcl-2 and Caspase-3 in mitochondrial apoptotic pathway in neonatal rat myocardial cells induced by high glucose and underlying mechanism. Methods: Left ventricule myocardial cells of neonatal SD rats were isolated and cultured with low-glucose culture media (LG group), low-glucose culture media added EGB (LG +EGB group), high-glucose culture media (HG group) and high-glucose culture media added EGB (HG+EGB group) in vitro respectively. The ratio of apoptosis of myocardial cells was analyzed by flow cytometry. The expression of Drp-1, Mfn-2, Bax, Bcl-2 and Caspase-3 were detected with western blot and/or RT-PCR respectively. Results: Compared to the LG group, the rate of apoptosis, the expression of Drp-1 and Bax, Caspase-3 and the ratio of Bax/Bcl-2 in ventricule myocardial cells were increased in HG group significantly (P<0.05), while the expression of Mfn-2 and Bcl-2 were decreased significantly (P<0.05). Compared to HG group, the rate of apoptosis, the expression of Drp-1 and Bax, Caspase-3 and the ratio of Bax/Bcl-2 in HG+EGB group were decreased significantly (P<0.05), while the expression of Bcl-2 was increased significantly (P<0.05). But the expression of Mfn-2 had no significant difference (P>0.05). Conclusion: EGB plays an important role in attenuation cardiomyocyte apoptosis induced by high glucose via its upregulation the expression of Bcl-2 and down regulation of Drp-1 and Bax, Caspase-3 and the ratio of Bax/Bcl-2. It may be one of the underlying mechanisms for EGB to treat diabetic cardiomyopathy.
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