The analysis of biological characristics of CD82 mediated human thyroid carcinoma FTC-133 cells
1.Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Research Group of Experimental and Surgical Oncology, Department of General, Visceral and Vascular Surgery, Martin Luther University Halle-Wittenberg, D-06097 Halle, Germany, 06102
CHEN Zhouxun1,ZHOU Hongzhong1,Henning Dralle2, et al. The analysis of biological characristics of CD82 mediated human thyroid carcinoma FTC-133 cells [J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(5): 346-.
Abstract：Objective: To study the role of tumor suppressor gene CD82 in the regulation of thyroid carcinoma cell invasion/migration and its related molecular mechanisms. Methods: By using genetic engineering technology, the plasmid pCDNA3.1, which reconsturcted with the full length CD82 gene, was transfected into the thyroid carcinoma FTC-133 cells. By MTT and Transwell Assays, the proliferation rate and migration ability of these high expressed CD82 FTC-133 cells were compared with the wild-type cells FTC-133 cells. Results: 24, 48, 72 hours after transfection, the transfected cells showed reduced proliferation rate (P>0.05) and significant lower migration ability (P<0.05) in comparison with the wild-type cells and mock cells (trasfected with empty plasmid). Conclusion: These results illustrate that CD82 can reduce FTC-133 cell proliferation and invasiveness, CD82 is closely related to development and progression of thyroid carcinoma.
