XU Changlong,WANG Jianzhang,ZHENG Bo, et al. Relationship between the methylenetetrahydrofolate reductase C677T, A1298C genetic polymorphisms and gastric cancer[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(5): 338-.
摘要目的：探讨亚甲基四氢叶酸还原酶（MTHFR）C677T和A1298C基因多态性与浙江汉族人群胃癌的关系。方法：采用聚合酶链反应-限制性片段长度多态性（PCR-RELP）技术，检测112例胃癌患者和218例正常对照者的MTHFR C677T及A1298C基因多态性分布差异。结果：MTHFR C677T的突变等位基因T及变异基因型（CT+TT）频率在胃癌组和对照组之间均无统计学差异（34.82% vs 32.57%和53.57% vs 52.75%，均P＞0.05）。胃癌组MTHFR A1298C突变等位基因C明显高于对照组，差异有统计学意义（21.43% vs 12.39%，P=0.006，OR=1.929，95%CI：1.325～2.873），而2组间突变基因型（AC+CC）频率差异无统计学意义（32.15% vs 36.24%，P＞0.05）。结论：浙江汉族人群MTHFR A1298C突变等位基因C可能与胃癌的易感性有关。
Abstract：Objective: To evaluate the association between methylenetetrahydrofolate reductase (MTHFR) C677T, A1298C genetic polymorphisms and gastric cancer in Zhejiang Han nationality population. Methods: Select 112 cases of patients with gastric cancer, choose health check-up 218 cases as controls. The genetic polymorphisms of MTHFR (C677T and A1298C) were detected by PCR-RELP method. Results: There were no significant discrepancies of the mutational allele T and variant genotype (CT+TT) frequencies in the MTHFR C677T gene between the gastric cancer patients and the controls (34.82% vs 32.57% and 53.57% vs 52.75%, respectively, all P>0.05). The frequency of mutational allele C in MTHFR A1298C gene was significantly higher in gastric cancer patients than that in the controls (21.43% vs 12.39%, P=0.006, OR=1.929, 95%CI: 1.325~2.873). However, there were no significant discrepancies of the variant genotype frequencies (AC+CC) between the two groups (32.15% vs 36.24%, P>0.05). Conclusion: The mutational allele C in MTHFR A1298C gene may be associated with the susceptibility to gastric cancer in Zhejiang Han nationality population.
