The effect of Qidong Huoxue Decoction on TLR4/NF- κB inflammatory signaling pathway in acute lung injury rats
1.Department of Respiration, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006; 2.Department of Respiration, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310005
HONG Huihua1,YANG Junchao1,GAO Rundi1, et al. The effect of Qidong Huoxue Decoction on TLR4/NF- κB inflammatory signaling pathway in acute lung injury rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(5): 332-.
Abstract：Objective: To investigate the protective effect of qidonghuoxue decoction (QD) on acute lung injury (ALI) in rats induced by lipopolysaccharide (LPS), and to explore the mechanism. Methods: Fifty SD rats were divided into five groups randomly: control group, model group, high-dose QD group, medium-dose QD group, low-dose QD group. The ALI model was established by intratracheal instillation of LPS. QD was administrated via esophagus to rats in QD groups; NS was substitution in control and model groups. All rats were executed 24 h after modeling, bronchoalveolar lavage fluid (BALF) and lung tissue were collected. Results: ①HE staining showed that QD could reduce alveolar damage, pulmonary edema and inflammatory cells infiltration in rats with ALI. ②TNF-α, IL-1β, IL-10 levels were increased in rats with ALI, QD decreased the levels of TNF-α and IL-1β, increased the level of IL-10, there was significant difference between the high-dose and low-dose group (P<0.01). ③The protein and mRNA expression of TLR4/NF-κB p65 both increased in rats with ALI, while QD reduced the expression,and the effect was kind of dose-related. Conclusion: QD has a protective effect on ALI induced by LPS in rats, its mechanism is related to inhibit TLR4/NF-kB p65 signaling pathway, decrease IL-1β/TNF-α levels and increase IL-10 level.
