MicroRNA-23a regulate the growth and migration of HUVECs in Kawasaki disease
1.Department of Cardiology, the Affiliated Changhai Hospital of Second Military University, Shanghai, 200433; 2.Department of Children’s Center, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 3.Children’s Heart Center, the Second Affiliated Hospital & Yuying Children’s Hospital, Institute of Cardiovascular Development & Translational Medicine, Wenzhou Medical University, Wenzhou, 325027
CHU Maoping1,HU Chen2,ZHOU Aihua2, et al. MicroRNA-23a regulate the growth and migration of HUVECs in Kawasaki disease[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(5): 321-.
Abstract：Objective: To study the effect of miR-23a that on the growth and migration of human umbilical vein endothelial cells (HUVECs) in Kawasaki disease. Methods: Real-time PCR was used to confirme miR-23a that differential expression between Kawasaki disease and controls. Bioinformatics analysis and the gene chip of inflammatory cytokine were used to target the genes. Transfect the miR-23a mimic/inhibitor in HUVECs.CCK-8 assay was used to measure the effect of proliferation. Cell scratch method and transwell chamber were performed to investigate the effect of migration. Results: MiR-23a was significantly higher in Kawasaki disease. The miR-23a mimic could promote the proliferation and migrate of HUVECs, the mi-23a inhibitor could inhibit the proliferation and migrate (P<0.05). Conclusion: The miR-23a play an important role in the development and progression of KD and could effect the proliferation and migrate of HUVECs.
