WANG Qiandong1,WU Shujuan2,ZHU Guowen1.. The biological impaction of morphine on lung cancer A549 cells induced by cisplatin[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(4): 269-.
Abstract：Objective: To analyze the effect and mechanism of the proliferation inhibition and apoptosis in human lung carcinoma after using morphine combined with cisplatin chemotherapy. Methods: Human lung adenocarcinoma cell line A549 was cultured in conventional 1 640 medium. Cell proliferation activity for cisplatin alone or in combination with morphine after treatment for 6 h, 12 h, 24 h and 48 h were detected by CCK8. In addition, Western Blot electrophoresis was adopted to detect cisplatin alone or in combination with the optimal concentration of morphine after 48 h. Then changes of apoptosis related proteins Bax, Bcl-2 Caspase-3, survivin expression level were analyzed. Results: After 24 h, or 48 h, the concentration of 500 μg/mL morphine in combination with cisplatin group, compared to morphine and cisplatin alone group, could significantly increase cancer cell proliferation inhibitory effect (P<0.05). While combined with low-dose concentrations of morphine-treated group, compared with cisplatin alone, the difference was not statistically significant. By WB experiments, we found that the combination of drug treatment could inhibit Bcl-2, survivin protein expression induced by cisplatin, while enhancing the pro-apoptosis death proteins Bax, Caspase-3 expression. Conclusion: The best choice for morphine dosage can significantly enhance proliferation and apoptosis function of cisplatin on lung cancer cell. This may inhibit the proliferation of lung cancer cells by promoting key apoptotic protein Bax and Caspase-3 expression pathway.
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