The role of microRNA-101a and cycloxygenase-2 in kidney damage induced by hyperuricemia
1.Department of Nephrology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 2.Department of Endocrinology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027
YE Hanyang1,JIN Jian2,LI Zhanyuan1, et al. The role of microRNA-101a and cycloxygenase-2 in kidney damage induced by hyperuricemia[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(4): 256-.
Abstract：Objective: To investigate the effects of miR-101a and cycloxygenase-2 (COX-2) on rats model of urate nephropathy. Methods: Twenty four rats models of urate nephropathy through fed with yeast and adenine for 21 days were divided into three groups, normal control groups, model groups and celecoxib groups. Serum creatinine (Cr), blood urea nitrogen (BUN) and blood uric acid (UA) was tested after feeding with yeast and adenine for 21 days. The real time PCR was preformed to analyze the expression of miR-101a and Western Blot was preformed to analyze the expression of COX-2 and caspase-3 in renal cortex. Results: Cr, BUN and UA of model group were significantly higher than that of normal control groups (P<0.05). The expression of miR-101a in renal cortex from model groups was lower than that of normal control groups (P<0.05), while higher than that of celecoxib groups (P<0.05). The expression of COX2 and caspase-3 in renal cortex from model groups were higher than that of normal control group (P<0.05), while lower than that of celecoxib groups (P<0.05). Conclusion: Decreasing miR-101a and increasing COX2 may involve in kidney damage induced by hyperuricemia, celecoxib as a specific inhibitor protects kidney from this damage.
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