Change of serum adiponectin, inflammation and oxidative stress in end-stage renal disease patients, influence of dialysis and its mechanisms
1.Department of Nephrology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 2.Department of Gastroenterology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 3.Clinical Laboratory, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027
CHEN Huile1,XU Changlong2,XU Xiaojie3, et al. Change of serum adiponectin, inflammation and oxidative stress in end-stage renal disease patients, influence of dialysis and its mechanisms[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(3): 190-.
Abstract：Objective: To explore change of the serum adiponectin (ADPN) levels, inflammation factor and oxidative stress in patients with end-stage renal disease (ESRD), influence of maintenance dialysis treatment and its mechanisms. Methods: Seventy-eight cases of patients with ESRD were selected and divided into non dialysis group (26 cases), hemodialysis group (27 cases) and peritoneal dialysis group (25 cases), another health check-up 30 cases were chosen as control group. By the methods of enzyme-linked immunosorbent assay (ELISA) serum adiponectin (ADPN), high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were detectd, the xanthine oxidase (XO) and myeloperoxidase (MPO) activity were detected with colorimetric method, superoxide dismutase (SOD) activity was detected with xanthine oxidase method, melondialdehyde (MDA) content was detected with glucosinolates barbituric acid method. Results: Three groups of patients with ESRD ADPN, hs-CRP, TNF-α, IL-6, XO, MPO and MDA were significantly higher than that of control group, and SOD were significantly lower than that of the control group, the difference was statistically significant (all P<0.01). Two dialysis groups, respectively, compared with non dialysis group, ADPN had no statistical difference (all P>0.05), and their hs-CRP, TNF-α, IL-6, XO, MPO and MDA were significantly higher than that of non dialysis group (P<0.05 or P<0.01), SOD were significantly lower than that of the non dialysis group (all P<0.01). Correlation analysis showed that ADPN with hs-CRP, TNF-α, IL-6, XO, MPO and MDA was significantly positive correlation (r=0.584, r=0.517, r=0.461, r=0.477, r=0.563, r=0.492, respectively, all P<0.01), and SOD was significantly negative correlation (r=-0.508, P<0.01). Conclusion: A significant rise in ESRD patients serum ADPN level, the main reason may be a decline in kidney function, inflammation and oxidative stress state mutual induction, synergy. Hemodialysis and peritoneal dialysis can not reduce the ADPN, while inflammation and oxidative stress aggravate.
