Effect of hepatitis B e antigen on the cytokine secretions and PI3K-Akt signaling pathway of mouse bone marrow-derived dendritic cells
1.Department of Gastroenterology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
WU Lecan1,WU Jinming1,LAN Songsong1, et al. Effect of hepatitis B e antigen on the cytokine secretions and PI3K-Akt signaling pathway of mouse bone marrow-derived dendritic cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(3): 171-.
Abstract：Objective: To investigate the effect of HBeAg on cytokine secretions and PI3K-Akt signaling pathway of mouse bone marrow-derived dendritic cells. Methods: The murine bone marrow cells were cultured and induced in vitro into DCs. Then the DCs were purified by murine CD11c microbeads and were matured overnight with LPS (100 ng/mL) to generate mature DCs. All the DCs were classified into three groups randomly, the control group, the OVA group and the HBeAg group. The secretions of IL-12p70 and IL-10 in DCs of all groups and the ability of three groups to stimulate allogenic T cell proliferation were detected by the enzyme-linked immunosorbent assay and the Mixed Lymphocyte Reaction, respectively. Then the western blot was used to detect the phosphorylation of Akt in three DCs groups. Results: After the HBeAg intervention, the expression of IL-12p70 in DCs and the stimulating T cell proliferation ability of DCs were significantly decreased compared to the other two groups (P<0.05), whereas the secretion of IL-10 and the phosphorylation of Akt in DCs were significantly increased (P<0.05). Conclusion: HBeAg may exploit PI3K-Akt signaling pathway as a strategy to suppress the immune response of DCs by inhibiting the secretion of IL-12 and increasing the secretion of IL-10.
