Effects of electroacupuncture and constraint-induced movement therapy on the expression of GAP-43 and GFAP after middle cerebral artery occlusion in rats
1.Department of Rehabilitation of Medical, Taizhou Hospital Affiliated to Wenzhou Medical University, Taizhou, 317000; 2.Department of Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027
FAN Liubo1,LIU Baohua2,ZHANG Xia1, et al. Effects of electroacupuncture and constraint-induced movement therapy on the expression of GAP-43 and GFAP after middle cerebral artery occlusion in rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(3): 166-.
Abstract：Objective: To observe the change of electroacupuncture and CIMT on the neural function and the expression of GAP-43 and GFAP after middle cerebral artery occlusion in rats and investigate the effect of electroacupuncture and CIMT on the neural function. Methods: MCAO was established by nylon monofilament. These rats were randomly divided into 4 group: model group, CIMT group, electroacupuncture group, and electroacupuncture with CIMT group. Then each group was randomly divided into 5 subgroups by 7 d, 14 d, 21 d, 28 d,
and 35 d. Meanwhile to design a sham-operation group. The corresponding treatment was performed respectively. The evaluations included the behavior scores and Morris water maze experiment, both was to compare the recovery of neural physiological function at different time points abovementioned. Immuno-histochemical technique was used to observe the positive cells of GAP-43 and GFAP in cortical ischaemic lesions of the rats in each group at different time points. The protein expression of GAP-43 and GFAP were detected by Western blot at day after ischemia. Results: The expression of GAP-43 in gray matter of rats were obviously increased in electroacupuncture with CIMT group compared with he model group, CIMT group, and acupuncture group after 14 d, 21 d,
28 d (P<0.05), The expression of GFAP in gray matter of rats were obviously decreased in electroacupuncture with CIMT group compared with CIMT group, electroacupuncture group after 14 d, 21 d, 28 d (P<0.05), but no significant difference was found between CIMT group and acupuncture therapy group after 35 d (P>0.05). Conclusion:
Electroacupuncture combined with CIMT can improve neurological function of focal cerebral ischemia rats, which may be associated with the increase of GAP-43 and descrease of GFAP in peri-infarct regions.
