Genetic polymorphism of nine short tandem repeat loci in han ethnic population in Wenzhou
1.Experiment Center of Basic Medicine, Wenzhou Medical University, Wenzhou, 325035; 2.Teaching Center of Biology Experiment of Wenzhou Medical University, Wenzhou, 325035; 3.Judicial Identification Center of Wenzhou Medical University, Wenzhou, 325035
WU Shuzhen1,3,ZHANG Hongqin2, et al. Genetic polymorphism of nine short tandem repeat loci in han ethnic population in Wenzhou[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(2): 123-.
摘要目的：分析温州汉族人群9个STR基因座（D18S1364、D12S391、D13S325、D6S1043、D2S1172、D11S2368、D22-GATA198B05、D8S1132、D7S3048）的等位基因及基因型频率分布。方法：从无血缘关系的355例温州汉族个体的抗凝血中提取DNA，用STR_Typer_10_v1试剂盒对9个STR基因座进行复合PCR扩增，用AB公司310遗传分析仪和GeneMapper ID 3.2v软件作STR分型，用PowerState V12.xls分析软件进行等位基因频率和法医学常用参数统计分析。结果：该9个基因座检出15、12、9、17、15、13、11、10、12个等位基因，9个基因座基因型频率分布均符合Hardy-Weignberg平衡（P＞0.05）；观测杂合度大于0.7831，多态信息含量均大于0.7666，个体识别能力（Dp）均大于0.9266。结论：温州汉族人群的9个基因座均具有较高的遗传多态性，是较理想的遗传标记系统，本研究所得数据可为温州汉族人群法医个体识别、亲权鉴定及遗传学研究提供依据。
Abstract：Objective: To analyze the alleles and genotype frequencies of nine short tandem repeats loci, those are D18S1364, D12S391, D13S325, D6S1043, D2S1172, D11S2368, D22-GATA198B05, D8S1132, D7S3048, of Han Ethnic Population in Wenzhou. Methods: extract DNA respectively from anticoagulant of 355 unrelated individual samples of Han Ethnic population living in Wenzhou. Deal the nine STR loci of the samples with multiple PCR amplification by STR_Typer_10_v1 kit. Analyze the PCR products by 310 genetic analyzer and GeneMapper ID 3.2v software of AB company. Deal the results with statistical analysis on the allele frequency and common forensic parameters by PowerState V12.xls software. Results: 15, 12, 9, 17, 15, 13, 11, 10 and 12 alleles were observed respectively from the nine STR loci. The distribution of genotype frequencies matched the Hardy-Weinberg equilibrium, P was more than 0.05. The heterozygote was more than 0.7831. The polymorphism information content was more than 0.7666. The combined power of discrimination was more than 0.9266. Conclusion: All of the nine STR loci of Han Ethnic Population in Wenzhou in this study have high genetic polymorphism, and it can be used as good genetic markers. The data obtained in this study can be used in individual identification, paternity test and population genetics investigation of Han Ethnic population living in Wenzhou.
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