Effects of lipopolysaccharide on proliferation and apoptosis in human umbilical vein endothelial progenitor cells
1.Department of General Surgery, the Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325200; 2.Department of General Surgery,Fuzhou General Hospital of Nanjing Military Command, Fuzhou, 350025
LI Jinhai1,CHEN Huichun1,DAI Huawei1, et al. Effects of lipopolysaccharide on proliferation and apoptosis in human umbilical vein endothelial progenitor cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(2): 115-.
Abstract：Objective: To investigate the effect of proliferation, apoptosis and cell cycle of lipopolysaccharide (LPS) on human umbilical vein endothelial progenitor cells. Methods: mononuclear cells were isolated from human umbilical cord blood. Mononuclearcells (MNCs) were isolated from human umbilical cord blood in vitro by Ficoll density gradient centrifugation. EPCs were characterized as adherent cells with double positive to DiI-acLDL uptake and lectin binding by direct fluorescent staining under a laser scanning confocal microscope. There were five groups. The control group and four LPS concentration groups: 2.5, 5.0, 10.0, 20.0 mmol/L. MTT was used to detect cell apoptosis and cell cycle. Results: ①10.0 mmol/L LPS promotes proliferation of EPCs, while 20.0 mmol/L LPS inhibits the proliferation of endothelial progenitor cells (P<0.05). ②20.0 mmol/L LPS promotes apoptosis of EPCs, compared with the control, the difference was significant (P<0.05). ③LPS at the concentration of 10.0 mmol/L reduces cells at G0/G1 phase and increases S and G2/M phase cells; 20.0 mmol/L LPS induces EPCs blockade at S phase, G2/M phase cells decreased, compared with the control, the difference was significant (P<0.05). Conclusion: 10.0 mmol/L LPS promotes the proliferation of EPCs. 20.0 mmol/L LPS inhibits the proliferation and promotes apoptosis of EPCs.
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