The role of changes of key enzymes of brain 5-hydroxytryptamine and 5-hydroxytryptamine bypass in rat model of postoperative fatigue syndrome
1.Department of Vascular Surgery, the First Affiliated Hospital of Wenzhou Medical University, Whenzhou, 325015; 2.Department of Anorectal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Whenzhou, 325015; 3.Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Whenzhou, 325015; 4.Department of General Surgery, Tenth People’s Hospital Affiliated to Tongji University, Shanghai, 200072
QIU Yihui1,YU Guanfeng1,YE Xingzhao2, et al. The role of changes of key enzymes of brain 5-hydroxytryptamine and 5-hydroxytryptamine bypass in rat model of postoperative fatigue syndrome[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(2): 85-.
Abstract：Objective: To explore the role of changes of key enzymes of brain 5-HT and 5-HT bypass in rat model of postoperative fatigue syndrome. Methods: 64 SD rats were randomly divided into model group and control group according to the weigh (which both including 1 d, 3 d, 7 d, 10 d group). The rats of model group would be resected the middle 70% small bowel, and control group only undergoing on-off abdominal surgery. The brains were removed on 1 d, 3 d, 7 d, 10 d postoperatively respectively, and we detected the concentration of 5-HT by high performance liquid chromatography and the mRNA expression of TPH1, TPH2, IDO by realtime fluores-cence quantitative PCR in hippocampus area. Results: Compared with control group, concentration of 5-HT of model group rats increased significantly on 1 d, 3 d postoperatively respectively (P<0.05). And in model group rats, concentration of 5-HT on 1 d, 3 d increased significantly respectively compared with 7 d, 10 d postoperatively (P<0.05), on 3 d to its highest (P<0.05), 7 d, 10 d gradually returned to normal. Compared with control group, mRNA expression of TPH1 in model group rats increased significantly on 3 d postoperatively (P<0.05). And in model group rats, mRNA expression of TPH1 on 3 d increased significantly compared with 1 d, 7 d, 10 d postoperatively (P<0.05). Compared with control group, mRNA expression of TPH2 in model group rats increased significantly on 1 d, 3 d postoperatively respectively (P<0.05). And in model group rats, mRNA expression of TPH2 on 1 d, 3 d increased significantly respectively compared with 7 d, 10 d postoperatively (P<0.05), on 3 d to its highest, 7 d, 10 d gradually returned to normal. Compared with control group, mRNA expression of IDO in model group rats increased significantly on 1d postoperatively (P<0.05). And in model group rats, mRNA expression of IDO on 1 d increased significantly compared with 3 d, 7 d, 10 d postoperatively (P<0.05). Conclusion: In rat model of postoperative fatigue syndrome, concentration of 5-HT increases significantly early postoperatively. Its mechanism refers to up-regulation of mRNA expression of TPH1, TPH2 of 5-HT rate-limiting enzyme.
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