Antioxidant protection against PC12 cells apoptosis induced by H2O2 and the preliminary mechanism of acylated 4-hydroxy chalcones
1.School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, 325035; 2.Chemical Biology Research Center, School of Pharmacy, Wenzhou Medical University, Wenzhou, 325035; 3.School of Sports Science, Wenzhou Medical University, Wenzhou, 325035
WANG Yanan1,SHEN Lailai2,WANG Zhankun3, et al. Antioxidant protection against PC12 cells apoptosis induced by H2O2 and the preliminary mechanism of acylated 4-hydroxy chalcones[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(1): 26-.
Abstract：Objective: To investigate the protective function of previously synthesized and screened chalcones B1 and B2 against PC12 cells apoptosis induced by H2O2, and their impact on Nrf2/ARE signaling pathway. Methods: A model that PC12 cells were damaged by H2O2 was established, and MTT assay was used to detect the antioxidant activity as well as cytotoxicity of compounds B1 and B2. Real-time PCR was used to determine mRNA expression of Nrf-2 regulated genes GCLC and HO-1. Hoechst staining was applied to test the inhibitory ability against H2O2-mediated PC12 cells apoptosis. Results: B1 and B2 showed no protection on PC12 cells induced by H2O2 in case of 1 hour incubation, while they represented potent antioxidant efficiency after incubation for 24 hours, and both were nontoxic at 10 μmol/L. B1 showed no obvious effect on the expression of GCLC and HO-1 which were Nrf2-regulated downstream genes. B2 significantly prevented the PC12 cells apoptosis caused by H2O2 with activating the expression of GCLC and HO-1. Conclusion: B1 and B2 show a significant antioxidant activity against H2O2-induced PC12 cells injury. The possible mechanism of B2 may be its activation of Nrf2/ARE signaling pathways while B1 may perform the antioxidant action via other route.
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