Effect of exogenous gonadotrophin on human fetal ovarian tissue after xenotransplantation
1.Department of Obstetrics and Gynecology, Jinhua Central Hospital, Jinhua, 321000; 2.Department of Reproduction Center, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000; 3.Anatomy Research Section, Jinhua Polytechnic, Jinhua, 321000
TONG Yuehong1,ZHOU Ying2,WU Dandan2, et al. Effect of exogenous gonadotrophin on human fetal ovarian tissue after xenotransplantation[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(1): 10-.
Abstract：Objective: To investigate the effect of exogenous gonadotrophin on the follicular development of cryopreserved human fetal ovarian tissue after xenotransplantation. Methods: The cryopreserved fetal ovarian tissues were grafted under kidney capsules of the nude mice. Mice randomly assigned to three different groups (A, B, C), and were treated with PMSG by different ways. Grafts of each group were recovered in batches at 10-14 weeks, 18-22 weeks, 26-30 weeks after operation. Grafts were evaluated using conventional histology and detected proliferating core antigen (PCNA) using immunohistochemical method. Results: There was no significant difference among three groups of preantral follicles. At 26-30 weeks, the numbers of secondary follicles in group C were significantly less than that in group A and B (P<0.05). Antral follicles were discovered in each group. Conclusion: The follices of the cryopreserved human fetal ovarian tissue can develop to antral follicle without exogenous hormone after xenotransplantation, and follicles can be promoted by exogenous gonadotropin.
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