Crebanine inhibits pancreatic cancer cell growth through DUSP5/ERK signaling pathway
LIN Chengyin1,XIANG Shixuan2, CHEN Letaotao3, DENG Jie4.
1.The First School of Medicine, School of Information and Engineering, Wenzhou Medical University, Wenzhou 325035, China; 2.Alberta Institute, Wenzhou Medical University, Wenzhou 325035, China; 3.Renji College, Wenzhou Medical University, Wenzhou 325035, China;4.Zhejiang Key Laboratory of Intelligent Cancer Biomarker Discovery and Translation, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325035, China
LIN Chengyin,XIANG Shixuan,CHEN Letaotao, et al. Crebanine inhibits pancreatic cancer cell growth through DUSP5/ERK signaling pathway[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2024, 54(8): 623-630.
Abstract：Objective: To explore the effect of crebanine on the proliferation and apoptosis of pancreatic cancer cells and its potential mechanism. Methods: CCK8, clone formation assay and EdU staining were used
to detect the effect of crebanine on the proliferation of human pancreatic cancer cells Panc-1 and Patu-8988. The effect of crebanine on apoptosis was detected by flow cytometry and TUNEL staining. Western blot assay was conducted to determine the effects of crebanine on DUSP5 protein, proliferation-related protein Ki67, apoptosisrelated proteins Bcl-2 and Bax, and p-ERK1/2. Results: After 24 h treatment with crebanine, the proliferation ability of pancreatic cancer cells decreased (P<0.05), while the apoptosis rate increased significantly (P<0.05).Meanwhile, the expression of DUSP5 was markedly upregulated (P<0.05), and the expression of p-ERK1/2 was downregulated (P<0.05). Knockdown of DUSP5 effectively reversed the anti-proliferation and pro-apoptotic abilities of crebanine in pancreatic cancer cells (all P<0.05), and partially restored the p-ERK1/2 protein reduced by crebanine (P<0.05). Conclusion: Crebanine inhibits the proliferation and promotes apoptosis of pancreatic cancer cells by upregulating DUSP5 expression and subsequently inactivating ERK1/2 signaling.
