Expressions and methylation analysis of apoptosis-related genes Survivin and Bcl2L13 in mice subchronic exposure to inhaled benzene
1.School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou, 325035; 2.School of Basic Medicine, Wenzhou Medical University, Wenzhou, 325035
LIN Chong1,LIU Zheng1,YU Xiuyuan1, et al. Expressions and methylation analysis of apoptosis-related genes Survivin and Bcl2L13 in mice subchronic exposure to inhaled benzene[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(7): 480-.
Abstract：Objective: To explore the expression level and the CpG islands methylation status of apoptosis-related genes Survivin and Bcl2L13 in mice submitted to subchronic inhalation of benzene. Methods: C57BL/6J mice were randomly divided into 3 groups: high concentration (100 ppm), low concentration (1 ppm) and control group. A subchronic benzene exposure test was carried out using the dynamic inhibition control equipment. At the end of exposure tests, bone marrow cells were collected. Cell cycle and apoptosis were detected by flow cytometry. DNA methylation status of promoter of Survivin and Bcl2L13 were determined using the Matrix-assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) and mRNA expressions were quantitatively analyzed using real-time PCR. Results: Compared with the control group, the percentages of apoptosis cells in both exposure groups were obviously higher (P<0.01). Cell cycle arrest at S phase was observed in low concentration group. The cell percentages at S phase and G2/M phase were significantly lower in high concentration group. Survivin mRNA expression was significantly reduced in low concentration group. Both Survivin and Bcl2L13 mRNA expressions were significantly suppressed in high concentration group. The low methylation levels of CpG islands of Survivin and Bcl2L13 promoters were observed in all groups. Moreover, the methylation levels were not impacted by benzene exposure. Conclusion: Subchronic exposure to benzene induced apoptosis, change the cell cycle, and decrease the mRNA expression of apoptosis-related genes Survivin and Bcl2L13 in mice bone marrow cell; but it don’t impact the methylation status of CpG islands in promoter regions of Survivin or Bcl2L13 genes.
