ZHU Tao,ZHAO Yingzheng. Design and synthesis of near-infrared embedded conjugated oligoelectrolytes and their application in
breast cancer-targeted imagining[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2024, 54(7): 574-582.
Abstract：Objective: To explore the application of near-infrared embedded conjugated oligoelectrolytes (COE) in the targeted imaging of breast cancer. Methods: Synthesize COE-BBT-2T and characterize the optical
properties of COE-BBT-2T using UV spectrophotometer and fluorescence spectrophotometer; Cytotoxicity
experiment (CCK8 experiment) was done to test the cytotoxicity of COE-BBT-2T; Prepare Lip-NK-COE; Laser particle size analyzer was used to test the particle size and potential of Lip-NK-COE; Transmission electron microscopy was used to capture the morphology of liposomes; Western blot was used to verify whether the extracted NK cell membrane was effectively embedded in liposomes, and laser confocal microscopy to verify whether Lip-NK-COE liposomes had the ability to target breast cancer cells (4T1 cells) in vitro; Animal experiments were divided into COE group, Lip-COE group and Lip-NK-COE group to detect the targeting and tumor imaging effect of Lip-NK-COE in breast cancer mice by near-infrared imaging in vivo. Results: The successfully synthesized COE-BBT-2T molecule shows near-infrared imaging performance and good biocompatibility. Lip-NK-COE was successfully prepared, the particle size being (101.7±2.1)nm, and the polydispersity index 0.224±0.040. The extracted NK cell membrane retained DNAM-1, NKG-2D cancer targeting related proteins, which was effectively embed into the liposome membrane. The targeting of Lip-NK-COE and 4T1 cells was detected to be stronger than that of Lip-COE through laser confocal microscopy, and the targeting of tumors in the Lip-NK-COE stronger than that in the Lip-COE using a near-infrared live imaging instrument.Conclusion: Lip-NK-COE can effectively target and image breast cancer.
