Exploring the mechanism of ursolic acid in the treatment of colon cancer based on the PI3K/Akt/mTOR
signaling pathway
CHEN Keke1, HONG Huang2, LIU Changbao1, CAO Weilan1.
1.Department of Anorectal Surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.Department of General Surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China
CHEN Keke,HONG Huang,LIU Changbao, et al. Exploring the mechanism of ursolic acid in the treatment of colon cancer based on the PI3K/Akt/mTOR
signaling pathway[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2024, 54(5): 396-400..
Abstract：Objective: To investigate the effect of ursolic acid on the colon cancer cells and its mechanism.Methods: A blank control group and different concentrations of ursolic acid treatment groups were established.The proliferation activity of each group of cells was measured using the CCK-8 method. The migration ability of each group of cells was measured through cell scratch assay, and the effect of ursolic acid on the PI3K/Akt/mTOR signaling pathway was measured through WB assay. Results: Through CCK-8 experiments, it was found that ursolic acid could significantly reduce the survival rate of SW480 and LOVO colon cancer cells, and with the increase of drug concentration, the survival rate of cells significantly decrease (P<0.05). The cell scratch experiment showed that compared with the control group, the low concentration of ursolic acid treatment did not significantly reduce the cell healing rate (P>0.05), while the high concentration of ursolic acid treatment significantly reduced the cell healing rate (P<0.05). The WB experiment results showed that the expression levels of p-PI3K, p-Akt, and p-mTOR proteins in cells treated with ursolic acid were significantly reduced (P<0.05).Conclusion: Ursolic acid has clear anti-tumor properties by inhibiting the expression of the PI3K/Akt/mTOR signaling pathway, thereby inhibiting the proliferation and migration of colon cancer cells.
