The role of miR-29a/b/c in uveal melanoma cells and its underlying mechanism
PAN Hongting1, 2, WANG Chao2, 3, ZHU Xian2, HOU Qiang1, 2.
1.School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, China; 2.State Key Laboratory of Optometry and Visual Science, Eye Hospital,Wenzhou Medical University, Wenzhou 325027, China; 3.Department of Pharmacy, Qionghai People’s Hospital,Qionghai 571400, China
Abstract：Objective: To investigate the role of miR-29a/b/c in uveal melanoma (UM) cells and the related mechanism. Methods: Hsa-miR-29a/b/c or matrix metalloproteinase 2 (MMP2)-specific siRNA were transfected
into UM cells by lipofectamine reagent. The proliferative and invasive potential of UM cells were evaluated by MTS and Matrigel Transwell assay respectively. MMP2 mRNA level was detected by Semi-quantitative PCR assay, and ELISA assay was used to measure the MMP2 secretion in the cell culture of supernatant of UM cells. Results: Transfection of miR-29a/b/c inhibited the proliferation (P<0.05) and invasive ability (P<0.05) of UM cells. MiR-29a/b/c could inhibit the expression of MMP2 at the protein level (P<0.05) instead of the mRNA level. Interfering MMP2 in UM cells inhibited the invasive ability (P<0.05) of UM cells. Conclusion: MiR-29a/b/ c inhibits the proliferative and invasive ability of UM cells. MiR-29a/b/c reduces the secretion of its downstream target protein MMP2, and the knockdown MMP2 in UM cells inhibits cell invasion.
