The effect of α-synuclein abnormal aggregation in red nucleus of mice on motor function and pain
SONGYue1, SU Ying2, HE Yan1, CHEN Jiangfan1, GUO Wei1.
1.School of Ophthalmology & Optometry, Biomedical Engineering, State Key Laboratory of Optometry and Vision Science, Wenzhou Medical University, Wenzhou 325027, China; 2.Key Laboratory of Pediatric Anesthesiology, Ministry of Education, Key Laboratory of Precision Anesthesiology of Zhejiang Province, Department of Anesthesiology and Perioperative Medicine, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China
SONGYue,SU Ying,HE Yan, et al. The effect of α-synuclein abnormal aggregation in red nucleus of mice on motor function and pain[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2024, 54(4): 259-265.
Abstract：Objective: To clarify whether abnormal accumulation of α-synuclein (α-Syn) in the red nucleus (RN) can disturb motor and sensory. Methods: C57BL/6J mice were randomly divided into two groups: the control group (injected with a control virus in RN) and the experimental group (injected with a virus expressing α-Syn in RN). The open field test, the Rotarod test, the pole test and the wire hang test were used to evaluate the mice’s motor ability and muscle strength. And the mechanical and thermal stimulations were used to evaluate the threshold of pain. Results: Compared with the control group, the experimental group showed abnormal aggregation of α-Syn in the RN, which significantly damaged neurons. For the experimental mice, their movement distance (P<0.001) and movement time (P<0.001) of in the open field significantly increased, while the immobility time significantly reduced (P<0.001); their latency of the drop significantly reduced in the Rotarod test (P<0.05); the time to arrive at the bottom of the turn increased in the pole test (P<0.05); the normalized limb muscle strength and weight in the grip strength test increased (P<0.05); and the thermal withdrawal latency significantly decreased (P<0.05). Conclusion: Abnormal aggregation of α-Syn in RN of mice promotes their spontaneous movement, reduces their balance and coordination abilities, enhances their limb muscle strength, and increases their sensitivity to thermal stimuli.
