ZHANG Yi,LI Feilei,DONG Jianda, et al. The mechanism of hsa_circ_0016600 promoting thyroid cancer cell migration via modulating the miR-149/PTK7 axis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2024, 54(2): 120-127，134.
Abstract：Objective: To investigate the impact of circular RNA hsa_circ_0016600 on the migration of thyroid cancer cells and its mechanisms. Methods: Cancer tissues and normal tissue specimens from 15 thyroid
cancer patients treated at the Department of Pate Surgery, the Second Affiliated Hospital of Wenzhou Medical University. Utilize RT-qPCR to assess the expression levels of hsa_circ_0016600, miR-149, and PTK7 in both thyroid cancer tissues and thyroid cell lines. Thyroid cancer cell lines were cultured in vitro, and knockdown experiments were conducted for hsa_circ_0016600 and overexpression experiments for miR-149. Cell proliferation, migration, and invasion capabilities were assessed using CCK-8 assays, transwell assays, and scratch assays. A xenograft subcutaneous tumor model in mice was established to observe the tumorigenic effect of thyroid cancer cells with hsa_circ_0016600 knockdown in vivo. Fluorescence in situ hybridization experiments were conducted to assess the expression level of hsa_circ_0016600 in the xenograft tumor model in nude mice. RNA fluorescence in situ hybridization experiments were performed to determine the cellular localization of hsa_circ_0016600 and miR-149 in thyroid cancer cells. Dual-luciferase reporter gene assays were used to investigate the targeted regulatory relationships between hsa_circ_0016600 and miR-149, as well as iR-149 and PTK7. Results: In human thyroid cancer tissues and cell lines, the expression of hsa_circ_0016600 (P<0.05)and PTK7 (P<0.01) was upregulated, while the expression of miR-149 (P<0.05) downregulated. Knockdown of hsa_circ_0016600 expression inhibited the proliferation (P<0.01), invasion (P<0.01), and migration (P<0.01)capabilities of thyroid cancer cells. Hsa_circ_0016600 could promote the proliferation (P<0.01), migration(P<0.01), and angiogenesis of thyroid cancer cells by targeting miR-149 and upregulating the expression of PTK7 (P<0.01). Additionally, PTK7 was a target gene of miR-149. Conclusion: Hsa_circ_0016600 is highly expressed in thyroid cancer cells and promotes thyroid cancer cell migration by targeting regulation of the miR-149/PTK7 axis.
