Regulatory effect of metformin on oxidative stress response in mouse hepatic ischemia/reperfusion injury
WANG Xinyu1, XU Junpeng1, WANG Xiaoting1, LIU Xiujie1, HUANG Qinghao2, CHEN Sian3, WANG Wantie1.
1.Institute of Ischemia/Reperfusion Injury, Wenzhou Medical University, Wenzhou 325035, China;2.Emergency Department of Liuzhou People’s Hospital, Liuzhou 545000, China; 3.The First School of Medicine,School of Information and Engineering, Wenzhou Medical University, Wenzhou 325035, China
WANG Xinyu,XU Junpeng,WANG Xiaoting, et al. Regulatory effect of metformin on oxidative stress response in mouse hepatic ischemia/reperfusion injury[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2024, 54(1): 47-52.
Abstract：Objective: To explore the effect of metformin on oxidative stress response in hepatic ischemia/reperfusion injury (HI/RI) in mouse. Methods: Fifteen SPF grade male C67BL/6 mice were randomly divided into three groups with 5 mice in each: the control group, the hepatic ischemia/reperfusion injury group (HI/RI group) and the hepatic ischemia/reperfusion injury+metformin group (MET group). The morphology of liver tissue was observed under the ordinary light microscopy; the degree of liver damage was evaluated with HE staining and PAS stain; the levels of ALT, AST, malondialdehyde (MDA) and Glutathione (GSH) were determined with a chemical kit. Results: Compared with the control group, the overall morphology, HE staining, and PAS staining of the HI/RI group showed an increase in the degree of liver tissue damage; The concentrations of ALT and AST in serum had a significant increase (P<0.01), so did the level of MAD in liver tissue (P<0.01), while the level of GSH significantly decreased (P<0.01); The red fluorescence intensity results in DHE staining showed a cumulative increase in ROS. Compared with the HI/RI group, the gross morphology, HE staining, and PAS staining of MET showed a significant reduction in liver tissue damage; The levels of ALT and AST in serum were significantly reduced (P<0.01), so did the MDA level in liver tissue (P<0.01), while the GSH level significantly increased (P<0.05); The DHE results showed a decrease in ROS accumulation. Conclusion: Metformin alleviates liver injury caused by ischemia/reperfusion, and it inhibits the oxidative stress response induced by liver ischemia/reperfusion.
