The effect of Notch3/DLL4 signal pathway on the lymphatic development after cystic hygroma
WANG Rongyue1, QIU Haifan1, DAI Fen1, LOU Wenwen2, GONG Jian2, XIE Ailan1.
1.Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China;2.Department of Laboratory Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China
WANG Rongyue,QIU Haifan,DAI Fen, et al. The effect of Notch3/DLL4 signal pathway on the lymphatic development after cystic hygroma[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2024, 54(1): 20-24,30.
Abstract：Objective: To investigate the effect of Notch3 signaling pathway on the maturation and development of fetal lymphatic vessels after cystic hygroma (CH). Methods: C57BL/6 mice and C57BL/6 background Notch3 gene knock-in mice were divided into a normal control group and a mutation group. Cervical lymph nodes were collected from F2 generation mice for HE staining, Masson staining, RT-qPCR, Western blot, and immunohistochemical analysis. Results: Compared with the control group. the results of HE and Masson staining showed that the cervical lymph nodes of the mutant mice were significantly disrupted and cellular fibrosis was severe; RT-qPCR results showed that the mRNA expression of DLL4, Hes1, and VEGFR3 in lymphatic nodes of Notch3 mutant mice was significantly decreased, which had statistical difference (P<0.05);Western blot analysis showed that the expression of DLL4, Hes1, and VEGFR3 proteins in the lymphoid nodes of Notch3 mutant mice was remarkably decreased, indicating significant difference (P<0.05);immunohistochemical analysis showed that the expression of DLL4, Hes1, Ang2, VEGFA, and VEGFR3 in the lymphoid nodes of Notch3 mutant mice was decreased, with significant difference (P<0.05). Conclusion:Notch3 regulates cystic hygroma in mice, and its mechanism is likely to be through lymphatic vessel neogenesis.
