ZHAO Jianguo,ZHU Xiaoling,JIN Xueying, et al. MiR-152-3p in the target regulation of KLF4 to promote proliferation, migration, and invasion in colorectal cancer cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(12): 954-962,968.
Abstract：Objective: To investigate the expression of microRNA-152-3p (miR-152-3p) in colon cancer (CC) and its effect of targeting Krüppel-like factor 4 (KLF4) on proliferation, migration and invasion of CC cells. Methods: The targeting relationship between miR-152-3p and KLF4 was predicted by starBase and TCGA database analysis, as well as the difference in their respective expression in normal and CC tissues. Dual luciferase and RIP experiments verified the targeting relationship between miR-152-3p and KLF4. The mRNA expression of miR-152-3p and KLF4 in CC cell lines was measured by RT-qPCR; the protein expression of KLF4 in CC cell lines was measured by Western blot; the proliferation ability of cells was examined by MTT; the migration ability of cells was examined by scratch healing assay; the invasion ability of cells was examined by Transwell assay; the cell cycle distribution and apoptosis percentage of cells were examined by cell cycle assay and apoptosis assay. Results: miR-152-3p was highly expressed in CC tissues and cell lines (P<0.001), while KLF4 was lowly expressed (P<0.01). miR-152-3p was able to target and downregulate KLF4 expression (P<0.01). miR-152-3p overexpression increased the proliferation, migration and invasion ability of CC cell lines, as well as cell cycle distribution in S and G2/M phases, and inhibited their apoptosis; KLF4 overexpression decreased the proliferation, migration and invasion ability of CC cell lines, contributing to cell cycle retention in G0/G1 phase, and accelerated apoptosis (P<0.05). The up-regulation of KLF4 expression inhibited the promotion of miR-152-3p on the proliferation, migration and invasion of CC cells (P<0.05), increased the proportion of cells in G0/G1 phase (P<0.05), and promoted apoptosis (P<0.05). Conclusion: miR-152-3p promotes proliferation,migration and invasion of CC cells by targeting down-regulation of KLF4 expression.
