Midline 2 expression upregulates in gastric cancer and promotes malignant phenotype of gastric cancer cells
CHEN Xietao1, HU Xianjing2, QIU Tianle1, SHEN Xian3, XUE Xiangyang1
1.Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Wenzhou 325035, China;2.Department of Anesthesiology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027,China; 3.Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015, China
CHEN Xietao,HU Xianjing,QIU Tianle, et al. Midline 2 expression upregulates in gastric cancer and promotes malignant phenotype of gastric cancer cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(11): 875-880，888.
Abstract：Objective: To analyze the expression level of midline 2 (MID2) in gastric cancer and its effect on
the prognosis of gastric cancer patients. To investigate the effect of MID2 expression on the biological phenotype of gastric cancer cells. Methods: A total of 420 tissue samples from the First Affiliated Hospital of Wenzhou Medical University after radical gastrectomy from January 2014 to December 2016 were retrospectively collected. The expression of MID2 in gastric cancer tissues and its effect on the prognosis of gastric cancer patients were evaluated by using gastric cancer tissue samples and TCGA database. Overexpression plasmids and small interfering RNAs (siRNAs) targeting MID2 genes were constructed, and the proliferation ability of gastric cancer cells was evaluated by CCK-8 experiment after transfection of gastric cancer cells while the migration and invasion ability of gastric cancer cells was evaluated by Transwell experiment. Results: The expression level of MID2 was upregulated in gastric cancer tissues (P<0.05). High MID2 expression predicted poorer overall survival (P=0.002), disease free survival (P=0.002) and progression free survival (P=0.003) in gastric cancer patients.Upregulating the expression of MID2 significantly promoted the proliferation, migration and invasion of gastric cancer cells BGC-823 and SGC-7901 (P<0.05). Knockdown MID2 could significantly inhibit the proliferation,migration and invasion of gastric cancer cells MGC-803 (P<0.05). Conclusion: The expression of MID2 in gastriccancer tissues is upregulated. High MID2 expression is associated with a poorer prognosis in patients with gastric cancer. Upregulating the expression of MID2 can significantly promote the malignant phenotype of gastric cancer cells.
