The effect of porcine acellular dermal matrix on fistula model repairing in rats and on TGF-β1/NF-κB p65 signaling pathway
LIN Qiongqiong1, MENG Hongbing2, JIN Chun3, ZHOU Chongjun3, SONG Zhangjuan4
1.Department of Pathology, the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027, China; 2.School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035,China; 3.Department of Anorectal Surgery, the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027, China; 4.Department of Training, Shanghai Pharmaceutical Vocational College, Shanghai 200092, China
LIN Qiongqiong,MENG Hongbing,JIN Chun, et al. The effect of porcine acellular dermal matrix on fistula model repairing in rats and on TGF-β1/NF-κB p65 signaling pathway[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(10): 801-806.
Abstract：Objective: To observe the effect of acellular dermal matrix on the repair of fistula model in rats and on TGF-β1/NF-κB p65 signaling pathway, and to explore its possible repairing mechanism. Methods: After adaptive feeding for one week, 40 healthy SD rats were randomly divided into four groups with 10 rats in each:sham operation group, model group, porcine acellular dermal matrix (pADM) group (dermal group), and positive control group (fistula plug group). After treatment for consecutive 2 weeks, the serum levels of IL-1β, IL-6,VEGF and prostaglandin E2 (PGE2) were measured by ELISA. The wound tissues were collected to observe the pathological changes. Western blot was used to detect the expression levels of TGF-β1, NF-κB p65 and VEGF protein. Results: Compared with the model group, the dermal group and fistula plug group increased significantly (P<0.05) in the body weight, and the wound pus decreased significantly. The levels of serum IL-1β, IL-6 and PGE2 in the dermal group were lower and the serum level of VEGF was higher (P<0.05). Meanwhile, HE staining showed that inflammatory infiltration in the dermal group reduced obviously. Moreover, the collagen fibers increased significantly and became denser. In both dermal group and fistula plug group, the expression levels of TGF-β1 and VEGF proteins increased while the expression of NF-κB p65 protein decreased in wound tissues (P<0.05). Conclusion: Porcine acellular dermal matrix could inhibit the inflammatory reaction in fistula wound tissue in rats, promoting angiogenesis and wound healing. Its promoting effect on collagen generation is much more obvious in the dermal group than in the anal fistula plug group. The underlying mechanism might be related to the regulation of the TGF-β1/NF-κB p65 signaling pathway.
