Effect of recombined granulocyte colony-stimulating factor on cerebral cortical proteins of ischemia-reperfusion injury rats
1.Department of Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 2.Department of Gerontology, the First Hospital of Jilin University, Changchun, 130021; 3.Department of Radiology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 4.Department of Neurology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027
LIU Baohua1,SHA Ying2,BAI Guanghui3, et al. Effect of recombined granulocyte colony-stimulating factor on cerebral cortical proteins of ischemia-reperfusion injury rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(5): 329-.
Abstract：Objective: To research the relation between difference protein with neuron protective effect of G-CSF in ischemia-reperfusion rats brain by using proteomics. Methods: Twenty-four experimental Wistar rats were divide into 3 groups, control group, rats cured by G-CSF group (G-CSF group), ischemia-reperfusion injured rats group (I/R group). Ischemia-reperfusion injury rats model was generated by using Koizumi’s way in G group and I/R group, one nylon thread was used to block the rats middle cerebral artery and reperfused after 2 hours. G-CSF (50 μg/kg/d) was injected subcutaneously on rats’ backs for successive 5 days in G-CSF group. Rats were executed and decapitated after 14 days after reperfusion to get the brain respectively. Sodium chloride injection was used in I/R group and control group. cortex proteins were extracted in the 3 groups of rats. Then the maps of the proteins were established by DIGE (differential gel electrophoresis, DIGE). The altered protein spots were identified with MALDI-TOF-MS and database searching. Results: Compared with the contrl group, the I/R group gained 56 differential protein spots, 39 spots expressed lowly, and 17 spots high. Compared with I/R group, the G-SCF group gained 35 differential protein spots, 16 spots expressed lowly, and 19 spots high, identified 6 protein spots, including dihydropyrimidinase-associated protein 2, glial fibrillary acidic protein, endomucin, Rho GDP dissociation inhibitor, Rab GDP dissociation inhibitor and guanine-nucleotide-binding protein. Conclusion: G-CSF is involved in neuroprotection after brain ischemia, possibly by regulating the expression of various neural regeneration-associated proteins at the subacute stage.
