LIANG Danna,LU Jianhua,YI Yongdong, et al. Preparation and functional exploration of polydopamine modified microspheres ALG@PDA[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(8): 613-620.
Abstract：Objective: To prepare polydopamine (PDA)-modified Calcium alginate microspheres (ALG@PDA microspheres) by microfluidic electrospray technology and dopamine (DA) self-polymerization, and to explore their properties. Methods: Calcium alginate microspheres (ALG microspheres) were prepared by microfluidic electrospray technology, and were modified by DA oxidation auto-polymerization. The particle size of microspheres was observed by the light microscope. Fourier transform infrared spectroscopy (FTIR) was used to detect the absorption peak of microspheres. The surface structure of the microspheres was observed by scanning electron microscopy. Near-infrared laser and thermal imager were used to explore the photothermal effect of microspheres. The viability of 3T3 cells treated with microsphere leaching solution was detected by the CCK-8 method and cell live/dead staining. Mouse tissues were used to test the adhesion ability of microspheres. Results: Within a certain range, the particle size of microcarriers was proportional to the concentration of precursor solution, the flow rate of precursor solution, and the inner diameter of capillary glass tube tip, but inversely proportional to the applied voltage. Based on the microfluidic electrospray technology and DA oxidation self-polymerization reaction, the injectable ALG@PDA with a particle size of about 200 μm and ALG@PDA has good biocompatibility. Thanks to the photothermal conversion ability of PDA, ALG@PDA had good photothermal effect compared with ALG alone, and ALG@PDA can be heated up to 21.5 ℃ in 3 min under NIR irradiation. Based on the plentiful catechol groups on the surface of PDA, compared with ALG alone, ALG@PDA had good adhesion ability, which could adhere to each other and to the tissue surface, thus increasing the residence time of ALG@PDA in the lesion. Conclusion: The prepared ALG@PDA microspheres have good biocompatibility, photothermal effect, and adhesion.
