The effect of basic fibroblast growth factor on liver injury in diabetic mice: A metabonomics analysis
YU Wenpiao1, SHU Qi2, WEI Tingting3.
1.The First School of Medicine, School of Information and Engineering,Wenzhou Medical University, Wenzhou 325035, China; 2.Department of Pharmacy, Zhejiang Cancer Hospital,Hangzhou 310022, China; 3.Laboratory Animal Research Center, Wenzhou Medical University, Whenzou 325035, China
YU Wenpiao,SHU Qi,WEI Tingting. The effect of basic fibroblast growth factor on liver injury in diabetic mice: A metabonomics analysis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(6): 458-464.
Abstract：Objective: To observe the effect of basic fibroblast growth factor (bFGF) on diabetic liver injury through remodeling metabolism by 1H nuclear magnetic resonance (1H NMR)-based metabonomics. Methods:C57BL/6J mice acted as control group (wt group). The db/db mice were randomly divided into diabetic liver injury group (db/db group) and bFGF-treated group (bFGF group). The liver tissues were collected from mice after 10 weeks of bFGF treatment. Morphology of liver tissue was examined by hematoxylin-eosin (HE) staining.The characteristic alterations in liver metabolites were explored by the projection to latent structure discriminant analysis (PLS-DA) based on the 1H NMR spectra. Results: HE staining results showed that the structure of hepatic lobules and hepatic sinuses in wt group was clear, and the steatosis of hepatic cells in db/db group was obvious. After bFGF intervention, lipid droplets and inflammatory cells in bFGF group were significantly reduced, and cell necrosis damage was relieved. PLS-DA results showed that there was significant difference in the metabolic profile of liver among the three groups of mice. Quantitative analysis of metabolites showed that,compared with age-matched wt group mice, the levels of leucine, isoleucine, valine, lactate, succinate, creatinine,glycine, guanosine triphosphate and phenylalanine were significantly lower in db/db group mice but they were significantly increased after bFGF treatment (P<0.05). Conclusion: The effect of bFGF on diabetic liver injury could be mediated by remodeling metabolic phenotype, mainly involving energy metabolism, amino acid metabolism and nucleotide metabolism.
