MEI Lei,TANG Qi,LI Liucen, et al. The effect of metformin on glioma cell metabolism under normoxic and hypoxic conditions[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(6): 440-449.
Abstract：Objective: To explore the effect of metformin on the metabolism of glioma U251 cells under normoxic and hypoxic conditions. Methods: Under the conditions of normox (21% O2) and hypoxia (1% O2),metformin at 25 mmol/L was used to detect the cell viability of U251 cells by MTT method; the ability of cells to form clones by plate cloning method; the DNA proliferation changes of cells were detected by EdU method;the cycle changes of cells were detected by cell cycle kits; the metabolic changes of cells were detected by ultra-high performance liquid chroma-high-resolution mass spectrometry untargeted metabolism detection. The content of ATP in cells and the content of extracellular lactic acid were detected by ATP and lactate kits, and the relative expression of glucose transporter (GLUT1) and proto-oncogene (c-Myc) proteins were detected by Western blot method, respectively. Results: Compared with the control group, the viability, cell formation cloning rate, cell proliferation rate and cell metabolite content were reduced (P<0.05) after 25 mmol/L metformin treated with glioma U251 cells for 48 h under normal oxygen and hypoxic conditions. Under normal oxygen conditions,metformin increased the ATP content and extracellular lactate content of brain glioma U251 cells (P<0.05).The relative expression of GLUT1 and c-Myc proteins in U251 cells after metformin treatment under hypoxic conditions decreased significantly (P<0.05). Conclusion: Metformin inhibits the proliferation of glioma U251 cells and the ability to form clones by downregulating the metabolism of glioma U251 cells.
