Changes of gut microbiota in ovariectomized rats based on amplicon and macrogene technology
DING Bingqian1, XU Linlin1, WANG Xueyuan1, ZHAO Xin1, WANG Yan1, FU Jinying1, 2.
1.He’nan University of Chinese Medicine, Zhengzhou 450002, China; 2.Department of Obstetrics and Gynecology, He’nan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou 450053, China
DING Bingqian,XU Linlin,WANG Xueyuan, et al. Changes of gut microbiota in ovariectomized rats based on amplicon and macrogene technology[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(5): 405-411.
Abstract：Objective: To investigate the changes of gut microbiota in ovariectomized rats before and after ovariectomy. Methods: Twelve 8-week-old SPF SD female experimental rats were fed adaptively for 3 days, and then the bilateral ovaries of 6 rats were randomly removed to replicate the clinical perimenopausal animal model.After that, 12 rats were divided as the control group (without any treatment) and the model group (with bilateral ovaries removed), which were fed in the same experimental environment for 3 weeks. Blood samples were collected from abdominal aorta and feces were taken. The changes of body weight and sex hormones in the two groups were observed, and the changes of gut microbiota were detected by 16S rDNA and macrogene technology. Results: Compared with the control group, the model group had significantly increased weight in experimental rats (P<0.05), significantly higher level of follicle-stimulating hormone and significantly lower level of estradiol (P<0.01), which confirmed the feasibility of perimenopausal animal model. The results of gut microbiota showed that the abundance of gut microbiota in the model group was significantly lower than that in the control group (P<0.05). Through KEGG functional annotation, the characteristic pathway of the model group was lipopolysaccharide biosynthesis pathway. Conclusion: A series of changes in perimenopausal syndrome are related to the decreased abundance of Limosilactobacillus reuteri, Lactobacillus acidophilus and uncultured Prevotella sp. and the signaling pathway of lipopolysaccharide biosynthesis.
