Effect of exercise inhibition HMGCS2 expression on alleviating mice cardiomyopathy induced by doxorubicin
CHEN Haili1, 2, SHEN Kangmin1, CHEN Yuewei1, WANG Li1, LU Junhong1, LI Shengcun1, 2, TU Wenzhan1, 2.
1.Rehabilitation Medical Center, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027,China; 2.Zhejiang Key Laboratory of Acupuncture and Moxibustion Rehabilitation and Wenzhou Key Laboratory of Rehabilitation, Wenzhou 325027, China
CHEN Haili,SHEN Kangmin,CHEN Yuewei, et al. Effect of exercise inhibition HMGCS2 expression on alleviating mice cardiomyopathy induced by doxorubicin[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(5): 386-391.
Abstract：Objective: To explore the effect of exercise on preventing myocardial atrophy and cardiac dysfunction after doxorubicin treatment. Methods: After swimming for 30 days and 40 minutes a day, the mice were divided into three groups, with 6 in each: the rest control group, and the exercise+doxorubicin group,with 6 mice in each group.The mouse model of doxorubicin was intraperitoneally injected with 20 mg/kg doxorubicin. Cardiac function of mice was detected with small animal ultrasound instrument VEVO2100 after 4 days, and then the myocardial tissue was taken for analysis. The cardiac function of the mice was detected with the small animal ultrasound instrument VEVO2100, and then the myocardial tissue was taken. The expression of atrophic markers Atrogin-1, MuRF-1 and HMGCS2 mRNA in myocardium was detected by qRT-PCR. The expression of HMGCS2 protein was detected by Western blot. Results: Doxorubicin induced myocardial atrophy and decreased cardiac function in mice (P<0.05); After doxorubicin injection, the expression of HMGCS protein and HMGCS2 mRNA in mouse myocardium increased (P<0.05); Exercise could prevent doxorubicin-induced cardiac dysfunction and myocardial atrophy (P<0.05). Exercise inhibited the expression of HMGCS2 in mice with doxorubicin cardiomyopathy (P<0.05). Inhibition of HMGCS2 in cultured myocardial cells could prevent doxorubicin-induced cardiomyocyte apoptosis (P<0.05). Conclusion:Exercise can protect cardiac function and myocardial atrophy in mice with doxorubicin cardiomyopathy by inhibiting HMGCS2.
