Myocardial atrophy and cardiac function in stroke mice improved by exercise preconditioning through upregulating Aplein/APJ
WANG Li1, 2, LU Junhong1, LIN Qijun1, GU Meilin1, TU Wenzhan1, 2, LI Shengcun1, 2.
1.Rehabilitation Medical Center, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027,China; 2.Zhejiang Key Laboratory of Acupuncture and Moxibustion Rehabilitation, Wenzhou Key Laboratory of Rehabilitation, Wenzhou 325027, China
WANG Li,LU Junhong,LIN Qijun, et al. Myocardial atrophy and cardiac function in stroke mice improved by exercise preconditioning through upregulating Aplein/APJ[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(5): 379-385.
Abstract：Objective: To explore the effect of exercise preconditioning on myocardial atrophy and cardiac function after stroke. Methods: The stroke mouse model was made by occlusion of middle cerebral artery, and the phenotype was observed. After 30 days of swimming exercise or resting, stroke mice models were created respectively. The expression of Atrogin-1, MuRF-1 and Apelin/APJ mRNA in myocardium was detected by qRTPCR.Small animal ultrasonic instrument was used to detect the cardiac function of mice, including an analysis of ejection fraction (EF), transient shortening rate (FS) and E/A ratio of diastolic function. The expression of APJ protein was detected by western blot. Results: Myocardial atrophy and decreased cardiac function occurred after stroke; The expression of Atrogin-1 and MuRF-1 in myocardium of stroke mice increased, while the expression of Apelin/APJ decreased (P<0.05). Exercise could reduce the expression of atrophy gene and myocardial atrophy but improve cardiac function after stroke. Exercise up-regulated the expression of Apelin/APJ in myocardium of stroke mice (P<0.05). Conclusion: Exercise preconditioning alleviated central muscle atrophy and decreased cardiac function in stroke. Exercise may prevent muscle atrophy in stroke center by up-regulating Apelin/APJ.
