Anti-gastric cancer activity of 4’-O-methylbroussochalcone B in vitro
TANG Yaling1, YI Baozhu2, LI Wulan3, WU Jianzhang4, ZHANG Huajie1.
1.School of Pharmaceutical Sciences, Wenzhou Medical University,Wenzhou 325035, China; 2.Department of Gynecology and Obstetrics, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China; 3.The First Clinical School of Medicine (School of Information and Engineering), Wenzhou Medical University, Wenzhou 325035, China;4.Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou 325101,China
TANG Yaling,YI Baozhu,LI Wulan, et al. Anti-gastric cancer activity of 4’-O-methylbroussochalcone B in vitro[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(3): 189-194.
Abstract：Objective: To investigate the anti-gastric cancer activity of 4’-O-methylbroussochalcone B in vitro. Methods: After the gastric cancer cell line AGS was treated with 4’-O-methylbroussochalcone B, the cell growth was detected by MTT and colony assay, cell migration by wounding healing assay, cell cycle and apoptosis by the flow cytometry, the expression of DYRK1A protein by Western blot. Results: The IC50 of 4’-Omethylbroussochalcone B on AGS was (7.7±1.3) μmol/L, which could inhibit colony formation and migration,induce cycle arrest and apoptosis, and down-regulate the expression of DYRK1A in a concentration-dependent manner (P<0.05). Conclusion: 4’-O-methylbroussochalcone B has significant anti-gastric cancer activity by inhibiting DYRK1A in vitro.
