YE Kaili,HUANG Shiqin,HU Ting, et al. Protective effect of butyrate on renal injury in diabetic nephropathy mice and its mechanism[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(1): 42-48.
Abstract：Objective: To investigate the protective effect of butyrate on renal injury in mice with type 2 diabetic nephropathy and to explore its mechanism. Methods: Male db/db mice were randomly divided into model group (DN), sodium butyrate 500 mg·kg-1·d-1 group (NaB1) and sodium butyrate 1 000 mg·kg-1·d-1 group (NaB2). The db/m mice with the same weeks served as the control group (NC). NaB1 and NaB2 groups were given sodium butyrate solution every day, while NC group and DN group were perfused with equal volume of physiological saline. After 8 weeks for intragastric administration, the mice were sacrificed. Kidney tissue was preserved for staining of hematoxylin eosin (HE) and periodic acid-Schiff (PAS); meanwhile extracting the mRNA and protein for real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting; using relevant kits to detect urine creatinine, urine microalbumin (mALB), interleukin-6 (IL-6) of renal tissue, serum creatinine (SCr) and other related indicators. Results: Compared with the NC group, the DN group presented listlessness, more polydipsia and polyuria, expressed significantly higher body weight and blood glucose (P<0.05), and increased the ratio of urinary protein and creatinine (UACR) (P<0.05). After the treatment of sodium butyrate for 8 weeks, the mice had better mental conditions and diet, lower blood glucose and UACR (P<0.05) and lower IL-6 levels of renal tissue, but higher expressions of GLP-1R, AMPK, PGC-1α, MFN2 and OPA1 mRNA (P<0.05). Conclusion: Sodium butyrate exerts reno-protective effect by regulating AMPK and GLP-1R in diabetic nephropathy mice.
