Expression characteristics of interleukin-10 and its impact on psoriasis
ZHENG Yi1, PAN Lina1, GAO Yu2
1.School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, China; 2.Department of Dermatology, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China
ZHENG Yi,PAN Lina,GAO Yu. Expression characteristics of interleukin-10 and its impact on psoriasis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2023, 53(1): 7-14.
Abstract：Objective: To analyze the characteristics of interleukin-10 (IL-10) in the skin lesions of psoriasis patients and psoriasis mouse models and its correlation with the severity of psoriasis, and to explore the effect of IL-10 expression on psoriasis. Methods: Samples of the skin lesions with psoriasis were collected from 40 patient between October 2018 and December 2019 in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, while psoriasis-causing models were induced by imiquimod (IMQ) in C57BL/6 (WT) mice and IL-10 knockout (IL-10-/-) mice. PASI score was used to observe the dynamic changes of skin lesions. Morphological changes in tissues, epidermal thickness and degree of inflammatory cell infiltration were observed by HE staining. Immunohistochemical staining to analyze protein expressions of IL-10 and CD19 in skin lesions. Immunofluorescence staining was used to analyze the changes of CD4 localization in skin lesions. Results: IMQ model could have skin manifestations similar to clinical psoriasis. The erythema score, scale score, skin infiltration thickness score, PASI total score and ear thickness of mice were significantly higher in the IL-10-/- model group compared with WT modeling group and IL-10-/- control group (P<0.05). Immunohistochemical results showed that the positive expression rate of IL-10 in skin lesions of the WT model group was lower than that of the WT control group, with significant difference (P<0.05). The positive expression rate of CD19 in mice in IL-10-/- model group was slightly higher than that in IL-10-/- control group and WT model group, with significant difference (P<0.05). Immunofluorescence results showed that CD4 positive expression in WT model mice spread from the epidermal-dermal junction to the whole epidermis, similar to the CD4 positive localization in psoriasis patient samples. CD4 positive expression was present in both epidermis and dermis of IL-10-/- model mice. Conclusion: IL-10 deficiency leads to sustained immune activation and exacerbates the onset and progression of psoriasis, which may aggravate psoriasis-like lesion changes by increasing scaling occurrence and promoting the proliferation and differentiation of keratin-forming cells in the epidermal layer of the skin.
