LI Hui,WANG Juan,YUAN Xinhua. The composition of intestinal flora in infants with functional constipation: an analysis based on 16S rDNA sequencing[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(12): 987-992.
Abstract：Objective: To explore the structure and composition of intestinal flora in infants with functional constipation by 16S rDNA sequencing and provide evidence for clinical treatment. Methods: From January to December 2020, 17 infants with functional constipation treated in Huzhou Maternity and Child Health Care Hospital were selected as the observation group, and 15 normal infants were assigned as the control group. With feces collected from the two groups, the 16S rDNA amplicon sequencing method was used to detect the intestinal bacteria to analyze the composition and compare the results. Results: There was no significant difference in the Alpha diversity of intestinal flora between the two groups (P>0.05), but there was significant difference in the Beta diversity (P<0.01). At the phylum level, Actinobacteria, Firmicutes, and Proteobacteria were the most abundant flora in the intestinal microbial communities of the two groups of children. Among them, the abundance of Verrucomicrobia in the constipation group was higher than that in the control group (P<0.05). At the genus level,Bifidobacterium was the dominant species in the two groups, and the abundance of Lactobacillus and Akkermansia in the intestinal tract of children with constipation was higher than that in the control group (P<0.05). The abundance of Bifidobacterium species, Microflora verrucosa, Achmania and Lactobacillus paracasei in the constipation group was statistically different, while that of Bifidobacterium dentatum, Actinomyces, Actinomycetidae,Actinomycetes and Lactobacillus rhamnosus in the control group was statistically different (P<0.05). Conclusion:There are changes in the structure and diversity of intestinal flora in children with functional constipation, There may be a correlation between constipation and intestinal flora.
